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Transformation of BALB/c-3T3 Cells: I. Investigation of Experimental Parameters That Influence Detection of Spontaneous Transformation
Edwin J. Matthews
Environmental Health Perspectives
Vol. 101, Supplement 2: Impact of the Environment on Reproductive Health (Jul., 1993), pp. 277-291
Published by: The National Institute of Environmental Health Sciences
Stable URL: http://www.jstor.org/stable/3431401
Page Count: 15
You can always find the topics here!Topics: Cultured cells, Seeding, Embryonic cells, Cell culture techniques, Transformed cell line, Surface areas, Chemicals, B lymphocytes, Phenotypes, Delta cells
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The frequency of spontaneous morphological transformation is an important variable in measuring chemical-induced transformation in BALB/c-3T3 clone A-31-1-13 cell cultures. Data from 110 experiments, which included benzo[a]pyrene control groups and other chemical treatment groups, were analyzed for factors that influenced spontaneous transformation. Spontaneous transformants demonstrated a continuum of morphological variants (type I, II, and III foci) that fit a normal distribution if converted to log10. The magnitude of transformation depended on the ampule of cryopreserved cells and the serum lot. Although the average frequency was approximately 0.71× 10-6 (type III foci/cell that survived and proliferated to confluence), the absolute number of foci/vessel increased in proportion to the surface area of the culture vessel. Thus, the frequency of spontaneous transformation was directly related to the cumulative number of mitoses that occurred in forming the contact-inhibited monolayer. These data are consistent with a hypothesis that spontaneous transformation in BALB/c-3T3 cells is a mutational event or some other single-step phenomenon.
Environmental Health Perspectives © 1993 The National Institute of Environmental Health Sciences