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Use of the Short-Term Inflammatory Response in the Mouse Peritoneal Cavity to Assess the Biological Activity of Leached Vitreous Fibers
K. Donaldson, J. Addison, B. G. Miller, R. T. Cullen and J. M. G. Davis
Environmental Health Perspectives
Vol. 102, Supplement 5: Biopersistence of Respirable Synthetic Fibers and Minerals (Oct., 1994), pp. 159-162
Published by: The National Institute of Environmental Health Sciences
Stable URL: http://www.jstor.org/stable/3432077
Page Count: 4
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We used a special-purpose glass microfiber sample, Johns-Manville Code 100/475, to study the effects of various acid and alkali treatments on biological activity as assessed by inflammation in the mouse peritoneal cavity, the leaching of Si, and the phase contrast optical microscopy (PCOM) fiber number. We used mild and medium treatments with oxalic acid and Tris buffer and harsh treatment with concentrated HCl and NaOH. Mild oxalic acid and Tris treatment for 2 weeks had no effect on any of the end-points, but prolonging the mild oxalic acid treatment time to 2 months reduced the biological activity and the fiber number. Medium oxalic acid treatment reduced the biological activity and the fiber number and caused a loss of Si. Medium Tris alkali treatment reduced the PCOM-countable fibers and the biological activity but did not cause a substantial loss of Si. Harsh treatment with strong HCl did not affect the fiber number or cause leaching but the biological activity was reduced; strong NaOH reduced the fiber number and biological activity, and caused marked leaching of Si. The medium oxalic acid conditions (pH 1.4) were more acid than those found in lung cells but produced the same effects (reduction in fiber number and biological activity) as the more physiological mild treatment (pH 4.0), when prolonged. This study suggests that medium oxalic acid treatment can be used as a short-term assay to compare loss of Si, reduction in fiber number, and change in biological activity of vitreous fibers. Such a combination of in vitro and in vivo assays is likely to provide the best approach to assessing the complex factors involved in changes in the toxicity of vitreous fibers caused by residence in the lung.
Environmental Health Perspectives © 1994 The National Institute of Environmental Health Sciences