Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

The E-Screen Assay: A Comparison of Different MCF7 Cell Stocks

Mercedes Villalobos, Nicolás Olea, José Antonio Brotons, María Fátima Olea-Serrano, J. Mariano Ruiz de Almodovar and Vicente Pedraza
Environmental Health Perspectives
Vol. 103, No. 9 (Sep., 1995), pp. 844-850
DOI: 10.2307/3432398
Stable URL: http://www.jstor.org/stable/3432398
Page Count: 7
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The E-Screen Assay: A Comparison of Different MCF7 Cell Stocks
Preview not available

Abstract

MCF7 human breast cancer cells have been studied extensively as a model for hormonal effects on breast cancer cell growth and specific protein synthesis. Because the proliferative effect of natural estrogen is considered the hallmark of estrogen action, it was proposed that this property be used to determine whether a substance is an estrogen. The E-screen assay, developed for this purpose, is based on the ability of MCF7 cells to proliferate in the presence of estrogens. The aim of our study was to characterize the response of four MCF7 cell stocks (BUS, ATCC, BB, and BB104) and determine which of them performed best in the E-screen test. The four stocks assayed were distinguishable by their biological behavior. In the absence of estrogen, MCF7 BUS cells stopped proliferating and accumulated in the G0/ G1 phase of the cell cycle; estrogen receptors increased, progesterone receptors decreased, and small amounts of pS2 protein were secreted. Of all the MCF7 stocks tested, MCF7 BUS cells showed the highest proliferative response to estradiol-17ß: cell yields increased up to sixfold over those of nontreated cells in a 144-hr period. The differences between estrogen-supplemented and nonsupplemented MCF7 BUS cells were due mostly to G0/ G1 proliferative arrest mediated by charcoal dextran-stripped serum. MCF7 BUS cell stocks and others showing a similar proliferative pattern should be chosen for use in the E-screen test, or whenever a proliferative effect of estrogen is to be demonstrated.

Page Thumbnails

  • Thumbnail: Page 
844
    844
  • Thumbnail: Page 
845
    845
  • Thumbnail: Page 
846
    846
  • Thumbnail: Page 
847
    847
  • Thumbnail: Page 
848
    848
  • Thumbnail: Page 
849
    849
  • Thumbnail: Page 
850
    850