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Rapidly and Slowly Replicating Human Immunodeficiency Virus Type 1 Isolates can be Distinguished according to Target-Cell Tropism in T-Cell and Monocyte Cell Lines
Stefan Schwartz, Barbara K. Felber, Eva-Maria Fenyö and George N. Pavlakis
Proceedings of the National Academy of Sciences of the United States of America
Vol. 86, No. 18 (Sep. 15, 1989), pp. 7200-7203
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/34428
Page Count: 4
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Human immunodeficiency virus type 1 (HIV-1) isolates from various patients were divided into two major groups, rapid/high and slow/low, according to their replication properties in vitro. Rapid/high isolates grow well in cell lines and induce the formation of syncytia in peripheral blood mononuclear cells. In contrast, slow/low isolates do not replicate in cell lines and rarely induce syncytia in peripheral blood mononuclear cells. To understand the differences in replicative capacity of these isolates, a panel of indicator cell lines was used. These cell lines were generated for sensitive detection of HIV-1 isolates and show characteristics of T-lymphoid or monocytoid cells. As a result of infection, chloramphenicol acetyltransferase expression is activated. Rapid/high viruses activate chloramphenicol acetyltransferase expression in T-cell and monocytoid indicator cell lines, whereas slow/low isolates activate chloramphenicol acetyltransferase expression only in monocytoid cell lines. The block in infection of T-lymphoid cells by the slow/low isolates appears to occur early in the infection cycle, prior to the production of the virally encoded tat protein. HIV-1 isolates can thus be distinguished according to target-cell tropism. Monocyte-derived cells seem to be a more general target for the various HIV-1 isolates.
Proceedings of the National Academy of Sciences of the United States of America © 1989 National Academy of Sciences