Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Effect of Gamma Radiation and Sex-Linked Recessive Lethal Mutations on Sperm Transfer in Ephestia kuehniella (Lepidoptera: Pyralidae)

J. Koudelová and P. A. Cook
The Florida Entomologist
Vol. 84, No. 2 (Jun., 2001), pp. 172-182
DOI: 10.2307/3496164
Stable URL: http://www.jstor.org/stable/3496164
Page Count: 11
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effect of Gamma Radiation and Sex-Linked Recessive Lethal Mutations on Sperm Transfer in Ephestia kuehniella (Lepidoptera: Pyralidae)
Preview not available

Abstract

Sperm quality and sperm competition play important roles in determining the efficiency of genetic methods for suppression of lepidopteran populations. Herein we have examined competitiveness of mutant and irradiated males of Ephestia kuehniella by counting eupyrene (fertile) and apyrene (non-fertile) sperm transferred to the female during copulation. Mutant BL-2 males, trans-heterozygous for two sex-linked recessive lethal mutations sl-2 and sl-15, produced 50% fewer of both types of sperm as compared to with WT-C (wild-type) males. However, the ratio of apyrene to eupyrene sperm remained the same in both male types (9.5:1). Irradiation of mature male pupae, heterozygous for either sl-2 or sl-15 mutations, with doses between 150 and 350 Gy showed dose-dependent effects on the amount of sperm transferred and on the total length of mating times. As the treatment dose increased the volume of sperm transferred by the male decreased and the mating times got longer. In the F1 descendants of the treated males, males were found to transfer either a relatively normal or a very small volume of sperm, which could reflect changes in gamete segregation and in chromosomal aberrations that are inherited. The dose of 175 Gy is suggested as optimal for irradiation of Ephestia kuehniella. /// La calidad y la competitividad del esperma es de suma importancia en la determinacion de la eficacia de los metodos geneticos destinados a la supresion de poblaciones de Lepidoptera. En este articulo hemos examinado la competitividad de machos de una sepa mutante asi como la competitividad de machos irradiados de la especie Ephestia kuehniella utilizando como indicador el numero total de esperma nucleado (eupyreno) y ancleado (apyreno) que estos machos transfieren durante la copula. Los machos de la sepa mutante (BL-2) que son trans-heterocigotos para dos mutaciones letales recesivas ligadas al sexo (sl-2 y sl-15), produjeron 50% menos cantidad de los dos tipos de esperma que los machos de tipo salvaje (WT-C). Sin embargo, la relacion entre la cantidad de esperma apyreno y eupyreno se mantuvo constante en ambos tipos de macho (9.5:1). La irradiacion de pupas maduras, heterocigotas para las mutaciones sl-2 o sl-15 con dosis entre 150 y 350 Gy mostro efectos dependientes de la dosis en cuanto a la cantidad de esperma transferido y el tiempo que los machos permanecen en copula. A medida que la dosis de irradiacion se incrementa, el volumen de esperma transferido decrece y el tiempo que los machos permanecen en copula aumenta. Asimismo, se encontro que los descendientes de machos irradiados (F1) transfieren ya sea una cantidad normal de esperma o una cantidad muy reducida de esperma lo cual indica que los cambios en la segregacion de gametos en cromosomas irradiados son heredados. La dosis de 175 Gy parece ser optima para la irradiacion de Ephestia kuehniella.

Page Thumbnails

  • Thumbnail: Page 
172
    172
  • Thumbnail: Page 
173
    173
  • Thumbnail: Page 
174
    174
  • Thumbnail: Page 
175
    175
  • Thumbnail: Page 
176
    176
  • Thumbnail: Page 
177
    177
  • Thumbnail: Page 
178
    178
  • Thumbnail: Page 
179
    179
  • Thumbnail: Page 
180
    180
  • Thumbnail: Page 
181
    181
  • Thumbnail: Page 
182
    182