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Immunoblot Technique for Q Fever (Technical Note)

H. Willems, D. Thiele, M. Glas-Adollah-Baik Kashi and H. Krauss
European Journal of Epidemiology
Vol. 8, No. 1 (Jan., 1992), pp. 103-107
Published by: Springer
Stable URL: http://www.jstor.org/stable/3520778
Page Count: 5
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Immunoblot Technique for Q Fever (Technical Note)
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Abstract

Partially purified but unfixed C. burnetii cells were submitted to sodium dodecylsulfate polyacrylamide gel electrophoresis, Western blotted and used as antigens for evaluation of antibody responses against the agent in mice, cattle and humans. Increased specificity was achieved by using glutaraldehyde as additional blocking agent. Gels as well as membranes were subjected to laser densitometry and data analysed with an IBM personal computer. This technique offers the possibility to objectively quantify and document the results of immunoblotting.

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