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Metaphase Chromosome Aberrations in Chinese Hamster Liver Cells in Vivo after Single Acute 60 Co Exposure

A. L. Brooks, R. F. Peters and M. D. Rollag
Radiation Research
Vol. 45, No. 1 (Jan., 1971), pp. 191-201
DOI: 10.2307/3573092
Stable URL: http://www.jstor.org/stable/3573092
Page Count: 11
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Metaphase Chromosome Aberrations in Chinese Hamster Liver Cells in Vivo after Single Acute 60 Co Exposure
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Abstract

The number and types of metaphase chromosomal aberrations induced by 60 Co gamma irradiation in the Chinese hamster liver have been determined. Partial hepatectomy was used to stimulate mitotic activity for chromosomal evaluation. Liver cells of the Chinese hamster exhibited similar sensitivities of 0.00060, 0.00087, and 0.00067 breaks/cell/R, respectively, to aberration induction by whole-body irradiation at 1 hour prior to (-1 hour) and 24 and 48 hours after partial hepatectomy. There was a shift from chromosome to chromatid aberrations which correlated closely with the biochemically determined onest of DNA synthesis. Dose-response curves for metaphase aberrations were established with the form $Y=a+bD+cD^{2}$ and coefficients of a = 0.01, $b=1.9\times 10^{-4}$, and $c=1.8\times 10^{-6}$. These indicated that liver cells were more resistant to radiation-induced aberrations than several other metaphase systems. The total number of aberrations per cell per roentgen was similar whether the animals were hepatectomized at 1 hour or 1 week after the radiation exposure. The frequency of aberrations increased as a function of time after partial hepatectomy. This was caused by an increase in deletions while the frequency of rings plus dicentrics remained constant. The advantages of scoring metaphase cells as compared to anaphase or telophase cells are discussed.

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