Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Interaction of Oxygen and Nitroxyls with Radiation-Induced Radicals of DNA and Related Bases in Aqueous Solution

P. O'Neill, T. C. Jenkins and E. M. Fielden
Radiation Research
Vol. 82, No. 1 (Apr., 1980), pp. 55-64
DOI: 10.2307/3575236
Stable URL: http://www.jstor.org/stable/3575236
Page Count: 10
  • Read Online (Free)
  • Download ($10.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Interaction of Oxygen and Nitroxyls with Radiation-Induced Radicals of DNA and Related Bases in Aqueous Solution
Preview not available

Abstract

The reaction of nitroxyl free radicals 2,2,6,6-tetramethylpiperidin-4-ol-N-oxyl (TMPN) and norpseudopelletierine-N-oxyl (NPPN) with peroxyl radicals derived from the · OH-adducts of DNA, thymine, and thymidylic acid has been investigated using pulse radiolysis. The results indicate that: (a) TMPN and NPPN react with the peroxyl radicals; (b) in the case of NPPN the resulting products, the optical absorption spectra of which were determined, are unstable and decay exponentially; and (c) native ${\rm DNA(OH)}^{\cdot}$ reacts with O2 with a rate constant $>10^{8}\ {\rm dm}^{3}\ {\rm mole}^{-1}\,{\rm sec}^{-1}$. In contrast to native DNA, where the optical absorption spectra for derived ${\rm DNA(OH)}^{\cdot}$ and ${\rm DNA}({\rm OH}){\rm O}{}_{2}{}^{\cdot}$ radicals are indistinguishable, denatured DNA affords analogous transient species the spectra of which differ markedly from one another.

Page Thumbnails

  • Thumbnail: Page 
55
    55
  • Thumbnail: Page 
56
    56
  • Thumbnail: Page 
57
    57
  • Thumbnail: Page 
58
    58
  • Thumbnail: Page 
59
    59
  • Thumbnail: Page 
60
    60
  • Thumbnail: Page 
61
    61
  • Thumbnail: Page 
62
    62
  • Thumbnail: Page 
63
    63
  • Thumbnail: Page 
64
    64