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DNA Damage Measured Using the Alkaline Elution Assay Depends on Time between Subculturing of Cells and Irradiation

Kathryn D. Held and Gary D. Bren
Radiation Research
Vol. 105, No. 2 (Feb., 1986), pp. 211-218
DOI: 10.2307/3576546
Stable URL: http://www.jstor.org/stable/3576546
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
DNA Damage Measured Using the Alkaline Elution Assay Depends on Time between Subculturing of Cells and Irradiation
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Abstract

In experiments utilizing the alkaline filter elution assay for radiation-induced DNA damage we observed an unexpected dependence of hypoxic dose-response curves on the length of time V79 cells were in exponential growth between subculturing and irradiation. Dose-response curves for DNA from cells irradiated in air were identical regardless of whether the exponential-phase cells had been subcultured 24 or 48 h prior to irradiation, but cells irradiated in hypoxia 24 h after subculture displayed a dose-response curve for DNA damage which was two times steeper than that obtained for cells irradiated in hypoxia 48 h after subculture. Possible mechanisms for this effect are discussed.

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