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The Effect of γ Radiation on DNA Methylation

John F. Kalinich, George N. Catravas and Stephen L. Snyder
Radiation Research
Vol. 117, No. 2 (Feb., 1989), pp. 185-197
DOI: 10.2307/3577319
Stable URL: http://www.jstor.org/stable/3577319
Page Count: 13
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The Effect of γ Radiation on DNA Methylation
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Abstract

The effect of 60 Co γ radiation on DNA methylation was studied in four cultured cell lines. In all cases a dose-dependent decrease in 5-methylcytosine was observed at 24, 48, and 72 h postexposure to 0.5-10 Gy. Nuclear DNA methyltransferase activity decreased while cytoplasmic activity increased in irradiated (10 Gy) V79A03 cells as compared to controls. No DNA demethylase activity was detected in the nuclei of control or irradiated V79A03 cells. Additionally, γ radiation resulted in the differentiation of C-1300 N1E-115 cells, a mouse neuroblastoma line, in a dose- and time-dependent manner. These results are consistent with the hypothesis that (1) genes may be turned on following radiation via a mechanism involving hypomethylation of cytosine and (2) radiation-induced hypomethylation results from decreased intranuclear levels of DNA methyltransferase.

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