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Changes in Bromodeoxyuridine Labeling Index during Radiation Treatment of an Experimental Tumor

J. Ramsay, H. D. Suit, F. I. Preffer and R. Sedlacek
Radiation Research
Vol. 116, No. 3 (Dec., 1988), pp. 453-461
DOI: 10.2307/3577388
Stable URL: http://www.jstor.org/stable/3577388
Page Count: 9
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Changes in Bromodeoxyuridine Labeling Index during Radiation Treatment of an Experimental Tumor
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Abstract

Cell proliferation kinetics in a spontaneous mouse fibrosarcoma (FSaII) growing in C3H mice has been studied by in vivo pulse labeling of cells synthesizing DNA with bromodeoxyuridine (BrdUrd). A monoclonal antibody to BrdUrd and flow cytometry were used to quantify these cells. Labeling indices (LI) were measured before and after radiation. Unirradiated 10-mm tumors had a mean LI of 17.5%. After a single dose of 20 Gy there was depression of LI after 1 day followed by a rapid increase to greater than control values after 5 days. Analysis performed after five fractions showed that LI was dependent on the dose per fraction and interval between fractions. After 5 and 7 Gy/fraction LI remained similar to control values during daily fractionation but was significantly depressed after twice daily fractionation. With doses greater than 10 Gy/fraction there was marked depression of LI using both fractionation schedules. These changes in LI correlated well with changes in tumor volume after radiation. Tumors were also biopsied after 5 fractions of a 20-fraction course to see if LI would predict for tumor control. LIs of ≥ 10% were associated with lack of tumor control at 90 days while all controlled tumors had a significant depression of LI. Changes in LI after radiation were a reasonable indication of the amount of repopulation occurring and might be useful in selecting patients for altered fractionation schedules.

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