You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Activation of Nuclear Factor κB in Human Lymphoblastoid Cells by Low-Dose Ionizing Radiation
Adapa V. Prasad, Natarajan Mohan, Bysani Chandrasekar and Martin L. Meltz
Vol. 138, No. 3 (Jun., 1994), pp. 367-372
Published by: Radiation Research Society
Stable URL: http://www.jstor.org/stable/3578685
Page Count: 6
You can always find the topics here!Topics: Ionizing radiation, Cell culture techniques, Gamma cells, Transcription factors, Cell lines, Radiation dosage, Dose response relationship, Cell nucleus, Genes, HeLa cells
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Nuclear factor κB (NF-κB) is a pleiotropic transcription factor which is involved in the transcriptional regulation of several specific genes. Recent reports demonstrated that ionizing radiation in the dose range of 2-50 Gy results in expression of NF-κB in human KG-1 myeloid leukemia cells and human B-lymphocyte precursor cells; the precise mechanism involved and the significance are not yet known. The present report demonstrates that even lower doses of ionizing radiation, 0.25-2.0 Gy, are capable of inducing expression of NF-κB in EBV-transformed 244B human lymphoblastoid cells. These results are in a dose range where the viability of the cells remains very high. After exposure to 137 Cs γ rays at a dose rate of 1.17 Gy/min, a maximum in expression of NF-κB was seen at 8 h after a 0.5-Gy exposure. Time-course studies revealed a biphasic time-dependent expression after 0.5-, 1- and 2-Gy exposures. However, for each time examined, the expression of NF-κB was maximum after the 0.5-Gy exposure. The expression of the p50 and p65 NF-κB subunits was also shown to be regulated differentially after exposures to 1.0 and 2.0 Gy.
Radiation Research © 1994 Radiation Research Society