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Radiosensitivity of CD45 RO+ Memory and ${\rm CD}45{\rm RO}^{-}$ Naive T Cells in Culture

Akiko Uzawa, Gen Suzuki, Yukiko Nakata, Makoto Akashi, Harumi Ohyama and Atsuo Akanuma
Radiation Research
Vol. 137, No. 1 (Jan., 1994), pp. 25-33
DOI: 10.2307/3578787
Stable URL: http://www.jstor.org/stable/3578787
Page Count: 9
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Radiosensitivity of  CD45 RO+ Memory and ${\rm CD}45{\rm RO}^{-}$ Naive T Cells in Culture
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Abstract

Radiosensitivities of various human T-cell subsets were investigated by a proliferation assay and by a single-cell gel electrophoresis assay. Each T-cell subset was purified using a cell sorter and was induced to proliferate by ionomycin and interleukin 2. Unsorted T cells showed biphasic dose-survival curves, indicating the heterogeneity of T cells in terms of radiosensitivity. Purified CD4+ helper and CD8+ killer T cells showed similar biphasic dose-survival curves. Hence both T-cell subsets were composed of cells of different radiosensitivity. The T-cell subsets belonging to different activation stages such as CD45 RO+ memory and ${\rm CD}45{\rm RO}^{-}$ naive T cells had different dose-survival curves. The former was more radiosensitive than the latter. The high radiosensitivity of CD45 RO+ cells was also demonstrated by single-cell gel electrophoresis after irradiation. This is the first demonstration that a particular cell surface marker on T cells is correlated with greater radiosensitivity.

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