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Inhibiting the Repair of DNA Damage Induced by Gamma Irradiation in Rat Thymocytes

J. A. Smit and J. H. Stark
Radiation Research
Vol. 137, No. 1 (Jan., 1994), pp. 84-88
DOI: 10.2307/3578794
Stable URL: http://www.jstor.org/stable/3578794
Page Count: 5
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Inhibiting the Repair of DNA Damage Induced by Gamma Irradiation in Rat Thymocytes
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Abstract

This study assessed the ability of 11 established and potential radiosensitizing agents to retard the repair of radiation-induced DNA damage with a view to enhancing the immunosuppressive effects of in vivo lymphoid irradiation. The capability of irradiated rat thymocytes to repair DNA damage was assessed by an adaptation of the fluorimetric unwinding method. Three compounds, 3-aminobenzamide (3-AB), novobiocin and flavone-8-acetic acid (FAA), inhibited repair significantly. We also report the effect of low-dose irradiation combined with repair inhibitors on the relationship between DNA strand breaks, fragmentation, cell viability and use of nicotinamide adenine dinucleotide (NAD). DNA fragmentation was increased by 1 mM/l FAA, 1 mM/l novobiocin and 50 μM/l RS-61443 within 3 h of incubation. The latter two compounds also proved cytotoxic. All three drugs augmented the effect of ionizing radiation on the use of NAD. Of the agents investigated, FAA showed the most promise for augmenting the immunosuppressive action of irradiation at nontoxic, pharmacokinetically achievable concentrations.

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