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Kinetics of Micronuclei Induced by ${}^{125}{\rm IdU}$ in Cells of Two Lines

G. Ludwików, F. Ludwików and K. J. Johanson
Radiation Research
Vol. 138, No. 1 (Apr., 1994), pp. 70-78
DOI: 10.2307/3578848
Stable URL: http://www.jstor.org/stable/3578848
Page Count: 9
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Kinetics of Micronuclei Induced by ${}^{125}{\rm IdU}$ in Cells of Two Lines
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Abstract

The kinetics of the formation of cells carrying micronuclei (MN) after one doubling time (t d) incorporation of ${}^{125}{\rm I}\text{-iododeoxyuridine}$ (${}^{125}{\rm IdU}$) to Chinese hamster ovary (CHO) and rat anterior pituitary tumor (GC) cells was studied. Uptake of ${}^{125}{\rm IdU}$ by cells of both cell lines was linearly dependent on the concentration of extracellular 125 I activity. The postlabeling timedependent decrease in cellular activity of ${}^{125}{\rm IdU}$ was exponential in CHO cells and approximately linear in GC cells. The maximum yield of MN was seen during the second and third t d after ${}^{125}{\rm IdU}$ incorporation. The frequency of cells with micronuclei increased monotonically with dose in the interval (1, 40) ${}^{125}{\rm I}\ \text{decays cell}^{-1}\ t_{{\rm d}}{}^{-1}$. The dose-response relationship could be fitted by straight lines with slopes of 1.0 (CHO) and 1.2 (GC) on the subinterval (1, 10) and of 0.6 or 0.5, respectively, for the subinterval (10, 40). Below one ${}^{125}{\rm I}\ \text{decay cell}^{-1}\ t_{{\rm d}}{}^{-1}$, the mean frequency of micronucleated binuclear cells was significantly lower than (CHO) or equal to (GC) the control. On average, one ${}^{125}{\rm I}\ \text{decay}/\text{cell}$ induced 0.95 ± 0.5% (CHO) or 1.0 ± 0.5% (GC) of micronucleated binuclear cells.

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