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Effects of Cell Cycle Position on Ionizing Radiation Mutagenesis. I. Quantitative Assays of Two Genetic Loci in a Human Lymphoblastoid Cell Line
Yao-Yu Chuang and Howard L. Liber
Vol. 146, No. 5 (Nov., 1996), pp. 494-500
Published by: Radiation Research Society
Stable URL: http://www.jstor.org/stable/3579549
Page Count: 7
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Relatively little work has been done on the influence of the position of the cell in the cell cycle on ionizing radiation-induced mutagenesis. We synchronized WTK1 human lymphoblastoid cells with 200 μM lovastain for 48 h; under these conditions more than 80% of the cells were arrested in G1 phase. Upon release, there was a 12-15-h lag followed by movement of a large fraction into S phase. We irradiated cells with either 1.5 Gy X rays at 1, 15, 18, 21 or 24 h or 1.5 Gy γ rays at 1, 5, 10, 15 or 24 h after release from lovastatin. We showed that WTK1 cells were most sensitive to ionizing radiation-induced toxicity in G1 and into S phase, and more resistant in mid to late S and G2/ M phase. Somewhat surprisingly, we found that the two different gene loci had different sensitivities to radiation-induced mutation through the cell cycle. Cells in late G1 through mid-S phase were most sensitive to radiation-induced mutations at the autosomal thymidine kinase (TK) locus, whereas G1 phase was the most sensitive phase at the X-linked hypoxanthine guanine phosphoribosyl transferase (HPRT) locus.
Radiation Research © 1996 Radiation Research Society