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Genetic Damage Induced by In Vitro Irradiation of Human G0 Lymphocytes with Low-Energy Protons (28 keV/μm): HPRT Mutations and Chromosome Aberrations

Maddalena Mognato, Elisabetta Bortoletto, Paola Ferraro, Laura Baggio, Roberto Cherubini, Sabrina Canova, Antonella Russo and Lucia Celotti
Radiation Research
Vol. 160, No. 1 (Jul., 2003), pp. 52-60
Stable URL: http://www.jstor.org/stable/3581234
Page Count: 9
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Genetic Damage Induced by In Vitro Irradiation of Human  G0 Lymphocytes with Low-Energy Protons (28 keV/μm): HPRT Mutations and Chromosome Aberrations
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Abstract

Cell survival, mutations and chromosomal effects were studied in primary human lymphocytes exposed in G0 phase to a proton beam with an incident energy of 0.88 MeV (incident LET of 28 keV/μm) in the dose range 0.125-2 Gy. The curves for survival and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were obtained by fitting the experimental data to linear and linear-quadratic equations, respectively. In the dose interval 0-1.5 Gy, the α parameters of the curves were 0.42/Gy and 3.6× 10-6 mutants/Gy, respectively. The mutation types at the HPRT locus were analyzed by multiplex-PCR in 94 irradiated and 41 nonirradiated clones derived from T lymphocytes from five healthy donors. All clones showed a normal multiplex-PCR pattern and were classified as point mutations. Chromosome aberration data were fitted as a linear function of dose (α = 0.62 aberrations per cell ${\rm Gy}^{-1}$). By irradiating G0 lymphocytes from a single subject with 28 keV/μm protons and γ rays, an RBE of 6.07 was obtained for chromosome aberrations. An overinvolvement of chromosome 9 relative to chromosome 7 was found in chromosome breaks after chromosome painting analysis.

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