You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Messenger RNA from Bovine Retina Induces Kainate and Glycine Receptors in Xenopus oocytes
I. Parker, K. Sumikawa and R. Miledi
Proceedings of the Royal Society of London. Series B, Biological Sciences
Vol. 225, No. 1238 (Jul. 22, 1985), pp. 99-106
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/35934
Page Count: 8
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
The retina contains several types of nerve cells that communicate through chemical synapses. The transmitter and receptor molecules that mediate signal transmission across these synapses need further characterization. For this purpose, poly (A)+ mRNA was isolated from bovine retinas and injected into Xenopus laevis oocytes. Translation of the foreign mRNA induced the oocyte membrane to acquire functional receptors to kainate and, to a lesser extent, also receptors to glycine, γ -aminobutryic acid (GABA), aspartate and glutamate. Thus, the cells in the retina must contain different messengers coding for these neurotransmitter receptors. Activation of the kainate receptors opens membrane channels, generating an ionic current which has an equilibrium potential close to 0 mv. The current is well maintained during prolonged application of kainate, and hence these receptors may be involved in the neurotoxic effects produced by kainate in the retina.
Proceedings of the Royal Society of London. Series B, Biological Sciences © 1985 Royal Society