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Molecular Systematics in the Genus Dictyota (Dictyotales, Phaeophyta): A First Attempt Based on Restriction Patterns of the Internal Transcribed Spacer 1 of the rDNA (ARDRA-ITS1)
Olivier De Clerck, Paul De Vos, Monique Gillis and Eric Coppejans
Systematics and Geography of Plants
Vol. 71, No. 1 (2001), pp. 25-35
Stable URL: http://www.jstor.org/stable/3668750
Page Count: 11
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This study represents a first attempt to reconstruct the phylogenetic relationships in the genus Dictyota by means of an RFLP analysis of the amplified Internal Transcribed Spacer 1 of the ribosomal region (ARDRA-ITS1). To overcome DNA extraction problems, a new protocol based on a combination of a CTAB method and a Sephaglass Bandprep Kit is presented. Seven species, mostly including more than one specimen, were included. Padina boergesenii was used as an outgroup. The inferred phylogenetic picture was congruent with the morphological-anatomical data. Specimens belonging to the same species invariably group together and differences among geographically isolated populations are reflected in the tree. Parsimony-based bootstrap and jackknife supports for basal nodes are generally very low and phylogenetic relationships between species are poorly resolved. This poor resolution can probably be attributed to the considerable variation in length of the ITS1 region among the species. This variation causes difficulties in sequence alignments and calls into question the use of this marker as a general tool in the genus.
Systematics and Geography of Plants © 2001 Botanic Garden Meise