Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Improved Media for Selecting Nitrate-Nonutilizing Mutants in Aspergillus flavus

Paul Bayman and Peter J. Cotty
Mycologia
Vol. 83, No. 3 (May - Jun., 1991), pp. 311-316
DOI: 10.2307/3759991
Stable URL: http://www.jstor.org/stable/3759991
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Improved Media for Selecting Nitrate-Nonutilizing Mutants in Aspergillus flavus
Preview not available

Abstract

Selection and identification of nitrate-nonutilizing (nit) mutants of Aspergillus flavus was compared on six chlorate-containing agar media. Three isolates of A. flavus were used. Nit mutants isolated comprised three phenotypic classes, equivalent to niaD, $nirA^{-}$, and cnx mutants of A. nidulans. There were significant differences among isolates and among media in number and types of nit mutants isolated. The most effective medium contained only nitrate as a nitrogen source, whereas most previously described media for isolation of nit mutants contain both nitrate and an amino acid. Morphology of nit mutants was more distinctive on this medium than on other media. The nitrate-only medium yielded mainly niaD mutants; other media yielded more $nirA^{-}$ mutants, which complement niaD mutants. Few cnx mutants were found. Use of a nitrate-only medium in conjunction with another medium should optimize isolation of nit mutants and complementary pairs of mutants.

Page Thumbnails

  • Thumbnail: Page 
311
    311
  • Thumbnail: Page 
312
    312
  • Thumbnail: Page 
313
    313
  • Thumbnail: Page 
314
    314
  • Thumbnail: Page 
315
    315
  • Thumbnail: Page 
316
    316