Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support

Cloning and Assignment of Linkage Group Loci to a Karyotypic Map of the Filamentous Fungus Aspergillus flavus

Kirk R. Foutz, Charles P. Woloshuk and Gary A. Payne
Mycologia
Vol. 87, No. 6 (Nov. - Dec., 1995), pp. 787-794
DOI: 10.2307/3760855
Stable URL: http://www.jstor.org/stable/3760855
Page Count: 8
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
If you need an accessible version of this item please contact JSTOR User Support
Cloning and Assignment of Linkage Group Loci to a Karyotypic Map of the Filamentous Fungus Aspergillus flavus
Preview not available

Abstract

The purpose of this study was to develop a karyotypic map of A. flavus and assign parasexually defined linkage groups to chromosomes. Seven A. flavus chromosomal bands, ranging in size from 2.3 to 7.0 megabases were separated by clamped homogeneous electric field gel electrophoresis. Polyethylene glycol-mediated transformation of A. flavus with a wildtype cosmid genomic library was used to complement seven strains having auxotrophic mutations in genes mapped to six of the eight linkage groups. DNA from cosmids complementing each of these seven mapped auxotrophic genes was used to make linkage group-specific probes. Hybridization of these probes to chromosomal separations allowed us to assign six linkage groups (I, II, IV, VI, VII, VIII) to five chromosomal bands. In addition, five previously cloned but unmapped genes were located on this karyotypic map and thus assigned to their corresponding linkage groups, i.e., benA, rDNA, pyrG, aflR and adh1 to groups I, II, II, VII, and IV or VIII, respectively. This karyotypic map will provide linkage group mapping of most cloned genes without parasexual analysis.

Page Thumbnails

  • Thumbnail: Page 
787
    787
  • Thumbnail: Page 
788
    788
  • Thumbnail: Page 
789
    789
  • Thumbnail: Page 
790
    790
  • Thumbnail: Page 
791
    791
  • Thumbnail: Page 
792
    792
  • Thumbnail: Page 
793
    793
  • Thumbnail: Page 
794
    794