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A Nomenclature for Fungal PCR Primers with Examples from Intron-Containing SSU rDNA

Andrea Gargas and Paula T. DePriest
Mycologia
Vol. 88, No. 5 (Sep. - Oct., 1996), pp. 745-748
DOI: 10.2307/3760969
Stable URL: http://www.jstor.org/stable/3760969
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Nomenclature for Fungal PCR Primers with Examples from Intron-Containing SSU rDNA
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Abstract

We present a compilation of polymerase chain reaction primers (oligonucleotides) used to amplify and sequence the small subunit of nuclear ribosomal DNA. To simplify use of these primers we designed a nomenclature that defines the location of each relative to Saccharomyces cerevisiae. With these primers we have developed strategies for selectively amplifying the diverse ribosomal DNA repeat types found in lichens; either between symbionts or between ribosomal DNA repeats (often the product of optional group I introns) within an individual fungus. A diversity of primers allows a choice of which small subunit ribosomal DNA repeat is amplified or sequenced from a complex extract such as that derived from lichens.

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