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Isolation and Characterization of the Single β-tubulin Gene in Phytophthora cinnamomi

N. D. Weerakoon, J. K. Roberts, L. P. Lehnen, Jr., J. M. Wilkinson and J. S. Marshall
Mycologia
Vol. 90, No. 1 (Jan. - Feb., 1998), pp. 85-95
DOI: 10.2307/3761016
Stable URL: http://www.jstor.org/stable/3761016
Page Count: 11
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Isolation and Characterization of the Single β-tubulin Gene in Phytophthora cinnamomi
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Abstract

To initiate studies on the regulation of tubulin expression and microtubule organization in the phytopathogenic Oomycete, Phytophthora cinnamomi, a gene encoding β-tubulin was cloned and sequenced. The gene has no introns and encodes a protein of 445 amino acids. Genomic Southern blots indicate a single copy of the β-tubulin gene in the P. cinnamomi genome. Comparison of the P. cinnamomi β-tubulin gene sequence with β-tubulin genes from a range of fungi, algae and protists supports the hypothesis that the Oomycetes are more closely related to the algae and protists than they are to the higher fungi.

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