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Potential of Spore Protein Profiles as Identification Tools for Arbuscular Mycorrhizal Fungi
Lisette J. C. Xavier, Ilungo J. Xavier and James J. Germida
Vol. 92, No. 6 (Nov. - Dec., 2000), pp. 1210-1213
Published by: Mycological Society of America
Stable URL: http://www.jstor.org/stable/3761488
Page Count: 4
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Five hundred spores from monospecific AMF cultures of Glomus clarum, G. mosseae, G. versiforme, G. fasciculatum, G. etunicatum, and a Glomus sp. isolate BVB1 were crushed in a modified sodium dodecyl sulphate (SDS) reducing buffer and 0.5 μg spore protein aliquots were subjected to one dimensional SDS polyacrylamide gel electrophoresis (1D SDS-PAGE). Silver-stained gels revealed a unique and reproducible protein profile for each of the AMF species tested. The average similarity between any two AMF species ranged ca 10-51%, as determined using an unweighted pair-group average approach. Furthermore, each of the AMF species possessed signature protein bands that were reproducible and consistent. SDS-PAGE of AMF spores is a simple and sensitive technique capable of distinguishing between AMF species that could be used for the routine identification of unknown AMF isolates.
Mycologia © 2000 Mycological Society of America