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Cephalosporium maydis Is a Distinct Species in the Gaeumannomyces-Harpophora Species Complex
Amgad A. Saleh and John F. Leslie
Vol. 96, No. 6 (Nov. - Dec., 2004), pp. 1294-1305
Published by: Mycological Society of America
Stable URL: http://www.jstor.org/stable/3762146
Page Count: 12
You can always find the topics here!Topics: Species, Acremonium, Mycology, Polymerase chain reaction, DNA, Histones, Biological taxonomies, Fungi, Ribosomal DNA, Corn
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Cephalosporium maydis is an important plant pathogen whose phylogenetic position relative to other fungi has not been established clearly. We compared strains of C. maydis, strains from several other plant-pathogenic Cephalosporium spp. and several possible relatives within the Gaeumannomyces-Harpophora species complex, to which C. maydis has been suggested to belong based on previous preliminary DNA sequence analyses. DNA sequences of nuclear genes encoding the rDNA ITS region, β-tubulin, histone H3, and MAT-2 support the hypothesis that C. maydis is a distinct taxon within the Gaeumannomyces-Harpophora species complex. Based on amplified fragment length polymorphism (AFLP) profiles, C. maydis also is distinct from the other tested species of Cephalosporium, Phialophora sensu lato and members of Gaeumannomyces-Harpophora species complex, which supports its classification as Harpophora maydis. Oligonucleotide primers for H. maydis were developed that can be used in a PCR diagnostic protocol to rapidly and reliably detect and identify this pathogen. These diagnostic PCR primers will aid the detection of H. maydis in diseased maize because this fungus can be difficult to detect and isolate, and the movement of authentic cultures may be limited by quarantine restrictions.
Mycologia © 2004 Mycological Society of America