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Spore Germination and Germ Tube Growth of a Vesicular-Arbuscular Mycorrhizal Fungus in vitro

J. O. Siqueira, D. H. Hubbell and N. C. Schenck
Mycologia
Vol. 74, No. 6 (Nov. - Dec., 1982), pp. 952-959
DOI: 10.2307/3792725
Stable URL: http://www.jstor.org/stable/3792725
Page Count: 8
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Spore Germination and Germ Tube Growth of a Vesicular-Arbuscular Mycorrhizal Fungus in vitro
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Abstract

The effects of mineral nutrients, thiamine, pH, and organic substrates on germination and germ tube growth of a vesicular-arbuscular mycorrhizal fungus were examined. Surface-sterilized azygospores of Gigaspora margarita were plated on 1% water agar containing different components and incubated at 28 C up to 30 da. Maximum germination and germ tube growth occurred when $1^{-1}\text{CaH}_{2}\text{PO}_{4}$ was used, whereas 24 mg $1^{-1}\text{NH}_{4}\text{NO}_{3}$ did not affect germination and depressed germ tube growth by 55%. Potassium or concentrations of soluble nutrient salts as high as those used to grow seedlings axenically had no effect on spore germination or germ tube growth. Thiamine at 1-1 increased germination, and maximum germ tube growth occurred at 1-1. The interaction between medium pH and medium composition was significant. Spore germination was not enhanced by any of the organic substrates. Low concentrations (4 g 1-1) of sucrose favored germ tube growth. Relation of these results to nutrition and root-colonizing ability of VA mycorrhizal spores is discussed.

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