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A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells

George H. Patterson and Jennifer Lippincott-Schwartz
Science
New Series, Vol. 297, No. 5588 (Sep. 13, 2002), pp. 1873-1877
Stable URL: http://www.jstor.org/stable/3832276
Page Count: 5
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A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
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Abstract

We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange.

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