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Stathmin-Tubulin Interaction Gradients in Motile and Mitotic Cells

Philipp Niethammer, Philippe Bastiaens and Eric Karsenti
Science
New Series, Vol. 303, No. 5665, Drug Discovery (Mar. 19, 2004), pp. 1862-1866
Stable URL: http://www.jstor.org/stable/3836517
Page Count: 5
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Abstract

The spatial organization of the microtubule cytoskeleton is thought to be directed by steady-state activity gradients of diffusible regulatory molecules. We visualized such intracellular gradients by monitoring the interaction between tubulin and a regulator of microtubule dynamics, stathmin, using a fluorescence resonance energy transfer (FRET) biosensor. These gradients were observed both during interphase in motile membrane protrusions and during mitosis around chromosomes, which suggests that a similar mechanism may contribute to the creation of polarized microtubule structures. These interaction patterns are likely to reflect phosphorylation of stathmin in these areas.

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