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Definition of a Bacterial Type IV Secretion Pathway for a DNA Substrate
Eric Cascales and Peter J. Christie
New Series, Vol. 304, No. 5674 (May 21, 2004), pp. 1170-1173
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/3836881
Page Count: 4
You can always find the topics here!Topics: Antibodies, DNA, Polymerase chain reaction, Adenosine triphosphatases, Secretion, Sexual reproduction, Cell extracts, Immunoprecipitation, Plants, Protein transport
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Bacteria use conjugation systems, a subfamily of the type IV secretion systems, to transfer DNA to recipient cells. Despite 50 years of research, the architecture and mechanism of action of the channel mediating DNA transfer across the bacterial cell envelope remains obscure. By use of a sensitive, quantifiable assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Agrobacterium tumefaciens. Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage.
Science © 2004 American Association for the Advancement of Science