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Activation of Endogenous Cdc42 Visualized in Living Cells
Perihan Nalbant, Louis Hodgson, Vadim Kraynov, Alexei Toutchkine and Klaus M. Hahn
New Series, Vol. 305, No. 5690 (Sep. 10, 2004), pp. 1615-1619
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/3837922
Page Count: 5
You can always find the topics here!Topics: Biosensing techniques, Dyes, Fluorescence, Pseudopodia, Human umbilical vein endothelial cells, Kinetics, Movies, Neutrophils, Physiological regulation, Line spectra
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Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.
Science © 2004 American Association for the Advancement of Science