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Bruchpilot Promotes Active Zone Assembly, Ca2+ Channel Clustering, and Vesicle Release

Robert J. Kittel, Carolin Wichmann, Tobias M. Rasse, Wernher Fouquet, Manuela Schmidt, Andreas Schmid, Dhananjay A. Wagh, Christian Pawlu, Robert R. Kellner, Katrin I. Willig, Stefan W. Hell, Erich Buchner, Manfred Heckmann and Stephan J. Sigrist
Science
New Series, Vol. 312, No. 5776 (May 19, 2006), pp. 1051-1054
Stable URL: http://www.jstor.org/stable/3846152
Page Count: 4
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Abstract

The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.

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