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Neutralization of TRIC Organisms by Antibody: Enhancement by Antisera Prepared against Immunoglobulins
W. A. Blyth and Janice Taverne
The Journal of Hygiene
Vol. 72, No. 1 (Feb., 1974), pp. 129-134
Published by: Cambridge University Press
Stable URL: http://www.jstor.org/stable/3862146
Page Count: 6
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The neutralizing activities of fowl, rabbit and mouse antisera prepared against TRIC agents were enhanced, in some cases over a 100-fold, by the addition of antiserum against the appropriate IgG. Significant non-specific inactivation of the organisms was observed in the absence of antiserum against TRIC agents, in reaction mixtures containing normal serum and antiserum against IgG. Since the extent of this inactivation showed a prozone, control tests with dilutions of normal serum equivalent to the full range of dilutions of the antiserum tested are necessary to show that enhancement of neutralization is specific.
The Journal of Hygiene © 1974 Cambridge University Press