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Salmonellas in Danish Pigs: A Comparison of Three Isolation Methods

Niels Skovgaard, Sven Gade Christensen and A. W. Gulistani
The Journal of Hygiene
Vol. 95, No. 1 (Aug., 1985), pp. 69-75
Stable URL: http://www.jstor.org/stable/3863056
Page Count: 7
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Salmonellas in Danish Pigs: A Comparison of Three Isolation Methods
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Abstract

Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller-Kaufmann medium (MK medium) with addition of 0·3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0·3% Teepol 610. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0·3% Teepol. (3) Incubation of 0·1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43 °C, subculture on BLSF agar. The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7·4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive. The number of salmonellas in the glands was low, probably less than ten per gram.

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