You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Usefulness of Ribotyping in a Molecular Epidemiology Study of Shigellosis
M. C. Mendoza, M. C. Martín and M. A. González-Hevia
Epidemiology and Infection
Vol. 116, No. 2 (Apr., 1996), pp. 127-135
Published by: Cambridge University Press
Stable URL: http://www.jstor.org/stable/3864609
Page Count: 9
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Ribotyping performed with six restriction endonucleases was used to study the molecular epidemiology of shigellosis in Asturias, Spain. The series included Shigella sonnei from 34 sporadic cases, 3 outbreaks and 3 reference strains, and S. flexneri from 13 sporadic cases and 1 reference strain. The S. sonnei strains were grouped into 5 ribotypes with Sal I, 4 with Hind III and Pvu II, 3 with Bgl II and EcoR I and 2 with Hinc II (Discriminatory Index (DI) between 0·54 and 0·14); the S. flexneri into 5 ribotypes with Sal I, Hinc II and Hind III, and 4 with the other enzymes (DI = 0·71-0·63). The combination of results for 2 or more enzymes facilitated an additional discrimination, the highest values in S. sonnei were for the 6 enzymes (16 types, DI = 0·91) and in S. flexneri for some combinations of 3 or more enzymes (7 types, DI = 0·81). Ribotypes with the 6 enzymes defined 16 clonal lines in S. sonnei and 7 in S. flexneri, which showed a different degree of genetic heterogeneity, and all the lines of each species falling into a different cluster. No line appeared as clearly endemic in the bowels of Asturian people.
Epidemiology and Infection © 1996 Cambridge University Press