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Nodulin Gene Expression and ENOD2 Localization in Effective, Nitrogen-Fixing and Ineffective, Bacteria-Free Nodules of Alfalfa
Clemens Van de Wiel, Joanna H. Norris, Birgit Bochenek, Rebecca Dickstein, Ton Bisseling and Ann M. Hirsch
The Plant Cell
Vol. 2, No. 10 (Oct., 1990), pp. 1009-1017
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/3869240
Page Count: 9
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Alfalfa plants form bacteria-free nodules in response to a number of agents, including Rhizobium meliloti exo mutants, Agrobacterium tumefaciens transconjugants carrying cloned R. meliloti nodulation genes, and compounds that function as auxin transport inhibitors, N-(1-naphthyl)phthalamic acid or 2,3,5-triiodobenzoic acid. These bacteriafree nodules contain transcripts for the nodulins Nms30 and MsENOD2; transcripts for late nudulins like leghemoglobin are not detected. In situ hybridization studies demonstrated that ENOD2 transcripts were localized in parenchyma cells at the base and along the periphery of nitrogen-fixing alfalfa root nodules. The ENOD2 gene was also expressed in a tissue-specific manner in nodules elicited by N-(1-naphtyl)phthalamic acid and 2,3,5-triiodobenzoic acid. In bacteria-free nodules induced by R. meliloti exo mutants and A. tumefaciens transconjugants carrying either one or both R. meliloti symbiotic plasmids, ENOD2 transcripts were also detected but were usually localized to parenchyma cells at the base instead of along the periphery of the nodule. On the basis of the pattern of ENOD2 gene expression, we conclude that the developmental pathway of bacteria-free nodules, whether bacterially or chemically induced, is the same as that of nitrogen-fixing nodules, and, furthermore, that the auxin transport inhibitors in their action mimic some factor(s) that trigger nodule development.
The Plant Cell © 1990 American Society of Plant Biologists (ASPB)