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Cold Calcium Signaling in Arabidopsis Involves Two Cellular Pools and a Change in Calcium Signature after Acclimation

Heather Knight, Anthony J. Trewavas and Marc R. Knight
The Plant Cell
Vol. 8, No. 3 (Mar., 1996), pp. 489-503
DOI: 10.2307/3870327
Stable URL: http://www.jstor.org/stable/3870327
Page Count: 15
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Cold Calcium Signaling in Arabidopsis Involves Two Cellular Pools and a Change in Calcium Signature after Acclimation
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Abstract

Cold shock elicits an immediate rise in cytosolic free calcium concentration ([ Ca2+] cyt) in both chilling-resistant Arabidopsis and chilling-sensitive tobacco (Nicotiana plumbaginifolia). In Arabidopsis, lanthanum or EGTA caused a partial inhibition of both cold shock [ Ca2+] cyt elevation and cold-dependent kin1 gene expression. This suggested that calcium influx plays a major role in the cold shock [ Ca2+] cyt response and that an intracellular calcium source also might be involved. To investigate whether the vacuole (the major intracellular calcium store in plants) is involved, we targeted the calcium-dependent photoprotein aequorin to the cytosolic face of the vacuolar membrane. Cold shock calcium kinetics in this microdomain were consistent with a cold-induced vacuolar release of calcium. Treatment with neomycin or lithium, which interferes with phosphoinositide cycling, resulted in cold shock [ Ca2+] cyt kinetics consistent with the involvement of inositol trisphosphate and inositide phosphate signaling in this response. We also investigated the effects of repeated and prolonged low temperature on cold shock [ Ca2+] cyt. Differences were observed between the responses of Arabidopsis and N. plumbaginifolia to repeated cold stimulation. Acclimation of Arabidopsis by pretreatment with cold or hydrogen peroxide caused a modified calcium signature to subsequent cold shock. This suggests that acclimation involves modification of plant calcium signaling to provide a "cold memory."

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