Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Effect of Pectin Methylesterase Gene Expression on Pea Root Development

Fushi Wen, Yanmin Zhu and Martha C. Hawes
The Plant Cell
Vol. 11, No. 6 (Jun., 1999), pp. 1129-1140
DOI: 10.2307/3870804
Stable URL: http://www.jstor.org/stable/3870804
Page Count: 12
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effect of Pectin Methylesterase Gene Expression on Pea Root Development
Preview not available

Abstract

Expression of an inducible gene with sequences common to genes encoding pectin methylesterase (PME) was found to be tightly correlated, both spatially and temporally, with border cell separation in pea root caps. Partial inhibition of the gene's expression by antisense mRNA in transgenic pea hairy roots prevented the normal separation of root border cells from the root tip into the external environment. This phenotype was correlated with an increase in extracellular pH, reduced root elongation, and altered cellular morphology. The translation product of the gene exhibited PME activity in vitro. These results are consistent with the long-standing hypothesis that the demethylation of pectin by PME plays a key role in cell wall metabolism.

Page Thumbnails

  • Thumbnail: Page 
[1129]
    [1129]
  • Thumbnail: Page 
1130
    1130
  • Thumbnail: Page 
1131
    1131
  • Thumbnail: Page 
1132
    1132
  • Thumbnail: Page 
1133
    1133
  • Thumbnail: Page 
1134
    1134
  • Thumbnail: Page 
1135
    1135
  • Thumbnail: Page 
1136
    1136
  • Thumbnail: Page 
1137
    1137
  • Thumbnail: Page 
1138
    1138
  • Thumbnail: Page 
1139
    1139
  • Thumbnail: Page 
1140
    1140