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tA Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta

Gregory T. Bryan, Kun-Sheng Wu, Leonard Farrall, Yulin Jia, Howard P. Hershey, Sean A. McAdams, Kristina N. Faulk, Gail K. Donaldson, Renato Tarchini and Barbara Valent
The Plant Cell
Vol. 12, No. 11 (Nov., 2000), pp. 2033-2045
DOI: 10.2307/3871103
Stable URL: http://www.jstor.org/stable/3871103
Page Count: 13
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
tA Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta
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Abstract

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent alleles of AVR-Pita. Using a map-based cloning strategy, we cloned Pi-ta, which is linked to the centromere of chromosome 12. Pi-ta encodes a predicted 928-amino acid cytoplasmic receptor with a centrally localized nucleotide binding site. A single-copy gene, Pi-ta shows low constitutive expression in both resistant and susceptible rice. Susceptible rice varieties contain $pi\text{-}ta^{-}$ alleles encoding predicted proteins that share a single amino acid difference relative to the Pi-ta resistance protein: serine instead of alanine at position 918. Transient expression in rice cells of a $Pi\text{-}ta^{+}$ R gene together with $AVR\text{-}Pita^{+}$ induces a resistance response. No resistance response is induced in transient assays that use a naturally occurring $pi\text{-}ta^{-}$ allele differing only by the serine at position 918. Rice varieties reported to have the linked $Pi\text{-}ta^{2}$ gene contain Pi-ta plus at least one other R gene, potentially explaining the broadened resistance spectrum of $Pi\text{-}ta^{2}$ relative to Pi-ta. Molecular cloning of the AVR-Pita and Pi-ta genes will aid in deployment of R genes for effective genetic control of rice blast disease.

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