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The ${\rm SCF}^{{\rm COI}1}$ Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis

Linghui Xu, Fuquan Liu, Esther Lechner, Pascal Genschik, William L. Crosby, Hong Ma, Wen Peng, Dafang Huang and Daoxin Xie
The Plant Cell
Vol. 14, No. 8 (Aug., 2002), pp. 1919-1935
Stable URL: http://www.jstor.org/stable/3871684
Page Count: 17
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The ${\rm SCF}^{{\rm COI}1}$ Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis
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Abstract

Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase complexes, which we have designated ${\rm SCF}^{{\rm COI}1}.{\rm COI}1_{{\rm E}22{\rm A}}$, a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the ${\rm SCF}^{{\rm COI}1}$ complexes and results in loss of the JA response. AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression. Furthermore, we show that the AtCUL1 component of ${\rm SCF}^{{\rm COI}1}$ complexes is modified in planta, where mutations in AXR1 decrease the abundance of the modified AtCUL1 of ${\rm SCF}^{{\rm COI}1}$ and lead to a reduction in JA response. Finally, we demonstrate that the axr1 and coi1 mutations display a synergistic genetic interaction in the double mutant. These results suggest that the COI1-mediated JA response is dependent on the ${\rm SCF}^{{\rm COI}1}$ complexes in Arabidopsis and that the AXR1-dependent modification of the AtCUL1 subunit of ${\rm SCF}^{{\rm COI}1}$ complexes is important for JA signaling.

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