Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Immunolocalization of Sarcoplasmic Reticulum Proteins in Mammalian Skeletal Muscle Fibers

Annelise O. Jorgensen
American Zoologist
Vol. 27, No. 4 (1987), pp. 1021-1032
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/3883317
Page Count: 12
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
Immunolocalization of Sarcoplasmic Reticulum Proteins in Mammalian Skeletal Muscle Fibers
Preview not available

Abstract

The sarcoplasmic reticulum is the intracellular membrane system in skeletal muscle fibers which regulates the Ca2+ concentration of the myofibril and thereby the contraction relaxation cycle. In the past the proposed explanation for the differences in the contractile properties of fast and slow skeletal fibers has been attributed mainly to quantitative rather than qualitative differences in the structure, function and molecular composition of the sarcoplasmic reticulum of these two fiber types. Recent immunocytochemical and biochemical studies have, however, clearly demostrated that the Ca2+- ATPase of the sarcoplasmic reticulum in slow skeletal fibers is structurally and thus perhaps also functionally related to that of the cardiac fibers, but distinctly different from that of fast skeletal fibers. Furthermore similar studies have shown that phospholamban, a cardiac sarcoplasmic reticulum protein believed to modulate the activity of the cardiac Ca2+- ATPase, is also present in slow but not fast skeletal fibers. The availability of antibodies specific to the fast and slow isoforms of the Ca2+- ATPase, and to phospholamban will now enable us to apply immunocytochemical labeling techniques to examine the effect of neuronal and other physiological signals on the regulation of the gene expression of sarcoplasmic reticulum proteins at the cellular level.

Page Thumbnails

  • Thumbnail: Page 
1021
    1021
  • Thumbnail: Page 
1022
    1022
  • Thumbnail: Page 
1023
    1023
  • Thumbnail: Page 
1024
    1024
  • Thumbnail: Page 
1025
    1025
  • Thumbnail: Page 
1026
    1026
  • Thumbnail: Page 
1027
    1027
  • Thumbnail: Page 
1028
    1028
  • Thumbnail: Page 
1029
    1029
  • Thumbnail: Page 
1030
    1030
  • Thumbnail: Page 
1031
    1031
  • Thumbnail: Page 
1032
    1032