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Journal Article

Protein and Mitochondrial DNA Variation in the Salamander Phaeognathus hubrichti

Mark L. McKnight, C. Kenneth Dodd, Jr. and Christina M. Spolsky
Herpetologica
Vol. 47, No. 4 (Dec., 1991), pp. 440-447
Published by: Allen Press on behalf of the Herpetologists' League
Stable URL: http://www.jstor.org/stable/3892849
Page Count: 8

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Topics: Mitochondrial DNA, Liver, Salamanders, Evolution, Dehydrogenases, Species, Enzymes, Herpetology, Fish, Genetic loci
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Protein and Mitochondrial DNA Variation in the Salamander Phaeognathus hubrichti
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Abstract

The genetic diversity and spatial distribution of selected genes of the Red Hills salamander, Phaeognathus hubrichti, were estimated by examination of variation in mitochondrial DNA (mtDNA) and proteins of 14 individuals collected across the entire range of the species. Digestion of mtDNA with each of 22 restriction enzymes resulted in fragment patterns that did not vary among these individuals. Nine of 35 protein loci were polymorphic; four of these showed a geographic pattern to the variation and suggest the existence of two forms in this threatened species. Preserves to protect the genetic diversity of this animal should include areas representing both forms.

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