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Conformational Disturbance in Abl Kinase upon Mutation and Deregulation
Roxana E. Lacob, Teodora Pene-Dumitrescu, Jianming Zhang, Nathanael S. Gray, Thomas E. Smithgall, John R. Engen and John Kuriyan
Proceedings of the National Academy of Sciences of the United States of America
Vol. 106, No. 5 (Feb. 3, 2009), pp. 1386-1391
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/40272387
Page Count: 6
You can always find the topics here!Topics: Deuterium, Crystal structure, Proteins, Hydrogen, Mass spectroscopy, Drug regulation, Molecules, Chronic myeloid leukemia, Genetic mutation, Amides
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Protein dynamics are inextricably linked to protein function but there are few techniques that allow protein dynamics to be conveniently interrogated. For example, mutations and translocations give rise to aberrant proteins such as Bcr-Abl where changes in protein conformation and dynamics are believed to result in deregulated kinase activity that provides the oncogenic signal in chronic myelogeous leukemia. Although crystal structures of the down-regulated c-Abl kinase core have been reported, the conformational impact of mutations that render Abl resistant to smallmolecule kinase inhibitors are largely unknown as is the allosteric interplay of the various regulatory elements of the protein. Hydrogen exchange mass spectrometry (HX MS) was used to compare the conformations of wild-type Abl with a nonmyristoylated form and with 3 clinically relevant imatinib resistance mutants (T315I, Y253H and E255V). A HX-resistant core localized to the interface between the SH2 and kinase domains, a region known to be important for maintaining the down-regulated state. Conformational differences upon demyristoylation were consistent with the SH2 domain moving to the top of the small lobe of the kinase domain as a function of activation. There were conformational changes in the T315I mutant but, surprisingly, no major changes in conformation were detected in either the Y253H or the E255V mutants. Taken together, these results provide evidence that allosteric interactions and conformational changes play a major role in Abl kinase regulation in solution. Similar analyses could be performed on any protein to provide mechanistic details about conformational changes and protein function.
Proceedings of the National Academy of Sciences of the United States of America © 2009 National Academy of Sciences