You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Independence of Metastatic Ability and Extravasation: Metastatic ras-Transformed and Control Fibroblasts Extravasate Equally Well
Sahadia Koop, Eric E. Schmidt, Ian C. MacDonald, Vincent L. Morris, Rama Khokha, Marsha Grattan, James Leone, Ann F. Chambers and Alan C. Groom
Proceedings of the National Academy of Sciences of the United States of America
Vol. 93, No. 20 (Oct. 1, 1996), pp. 11080-11084
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/40286
Page Count: 5
You can always find the topics here!Topics: 3T3 cells, Fibroblasts, NIH 3T3 cells, Arterioles, Cells, Embryos, Capillaries, Microvessels, Venules, Metastasis
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Escape of cancer cells from the circulation (extravasation) is thought to be a major rate-limiting step in metastasis, with few cells being able to extravasate. Furthermore, highly metastatic cells are believed to extravasate more readily than poorly metastatic cells. We assessed in vivo the extravasation ability of highly metastatic ras-transformed NIH 3T3 cells (PAP2) versus control nontumorigenic nontransformed NIH 3T3 cells and primary mouse embryo fibroblasts. Fluorescently labeled cells were injected intravenously into chicken embryo chorioallantoic membrane and analyzed by intravital videomicroscopy. The chorioallantoic membrane is an appropriate model for studying extravasation, since, at the embryonic stage used, the microvasculature exhibits a continuous basement membrane and adult permeability properties. The kinetics of extravasation were assessed by determining whether individual cells (n = 1481) were intravascular, extravascular, or in the process of extravasation, at 3, 6, and 24 h after injection. Contrary to expectations, our results showed that all three cell types extravasated with the same kinetics. By 24 h after injection >89% of observed cells had completed extravasation from the capillary plexus. After extravasation, individual fibroblasts of all cell types demonstrated preferential migration within the mesenchymal layer toward arterioles, not to venules or lymphatics. Thus in this model and for these cells, extravasation is independent of metastatic ability. This suggests that the ability to extravasate in vivo is not necessarily predictive of subsequent metastasis formation, and that postextravasation events may be key determinants in metastasis.
Proceedings of the National Academy of Sciences of the United States of America © 1996 National Academy of Sciences