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Cohesin SMC1β Protects Telomeres in Meiocytes

Caroline Adelfalk, Johannes Janschek, Ekaterina Revenkova, Cornelia Blei, Bodo Liebe, Eva Göb, Manfred Alsheimer, Ricardo Benavente, Esther de Boer, Ivana Novak, Christer Höög, Harry Scherthan and Rolf Jessberger
The Journal of Cell Biology
Vol. 187, No. 2 (Oct. 19, 2009), pp. 185-199
Stable URL: http://www.jstor.org/stable/40384466
Page Count: 15
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Cohesin SMC1β Protects Telomeres in Meiocytes
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Abstract

Meiosis-specific mammalian cohesin SMC1β is required for complete sister chromatid cohesion and proper axes/loop structure of axial elements (AEs) and synaptonemal complexes (SCs). During prophase I,telomeres attach to the nuclear envelope (NE), but in$Smc1\beta ^{ - / - } $ meiocytes, one fifth of their telomeres fail to attach. This study reveals that SMC1β a serves a specific role at telomeres, which is independent of its role in determining AE/SC length and loop extension. SMC1β is necessary to prevent telomere shortening, and SMC3, present in all known cohesin complexes, properly localizes to telomeres only if SMC1β is present.Very prominently, telomeres in $Smc1\beta ^{ - / - }$ spermatocytes and oocytes loose their structural integrity and suffer a range of abnormalities. These include disconnection from SCs and formation of large telomeric protein-DNA extensions, extended telomere bridges between SCs, ring-like chromosomes, intrachromosomal telomeric repeats, and a reduction of SUN1 foci in the NE. We suggest that a telomere structure protected from DNA rearrangements depends on SMC1β.

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