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A Rapid Method for Enumeration of Viable Seeds in Soil

Charles R. Malone
Weeds
Vol. 15, No. 4 (Oct., 1967), pp. 381-382
DOI: 10.2307/4041016
Stable URL: http://www.jstor.org/stable/4041016
Page Count: 2
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Abstract

Soil dispersion in an aqueous solution of sodium hexametaphosphate and sodium bicarbonate facilitates extraction of seeds by flotation with megnesium sulphate. The percentage viability of seeds collected in this manner subsequently can be determined with 2,3,5-triphenyl-tetrazolium chloride. Extraction of seeds usually is 100% efficient and enumeration of viable seeds via the tetrazolium test is accomplished much more rapidly than with conventional methods.

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