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The Biosynthesis of N-(3-Carboxy-2,5-Dichlorophenyl)-Glucosylamine in Plant Tissue Sections

D. S. Frear, C. R. Swanson and R. E. Kadunce
Weeds
Vol. 15, No. 2 (Apr., 1967), pp. 101-104
DOI: 10.2307/4041173
Stable URL: http://www.jstor.org/stable/4041173
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Biosynthesis of N-(3-Carboxy-2,5-Dichlorophenyl)-Glucosylamine in Plant Tissue Sections
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Abstract

A quantitative colorimetric procedure was developed for the determination of N-glucosyl amiben in tissue sections of etiolated seedlings. N-glucosyl amiben biosynthesis in soybean hypocotyl sections was heat labile, sensitive to freezing and thawing, unaffected under anaerobic conditions, dependent on amiben concentration, and proportional to tissue section concentration. The formation of N-glucosyl amiben in soybean hypocotyl sections was greatest between pH 6.3 and pH 7.3 and was inhibited by NaF, HgCl₂, iodoacetate, iodoacetamide, and N-ethylmaleimide but not by NaCN or NaN₃. On a specific activity basis, sugar beet seedling, soybean cotyledon, and soybean hypocotyl tissue sections were the most active tissues studied in the biosynthesis of N-glucosyl amiben.

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