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Silver Ion High Pressure Liquid Chromatography Provides Unprecedented Separation of Sterols: Application to the Enzymatic Formation of cholesta-5,8-dien-3$\beta $-ol

Benfang Ruan, Justin Shey, Nicolas Gerst, William K. Wilson and George J. Schroepfer
Proceedings of the National Academy of Sciences of the United States of America
Vol. 93, No. 21 (Oct. 15, 1996), pp. 11603-11608
Stable URL: http://www.jstor.org/stable/40481
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Silver Ion High Pressure Liquid Chromatography Provides Unprecedented Separation of Sterols: Application to the Enzymatic Formation of cholesta-5,8-dien-3$\beta $-ol
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Abstract

We report that silver ion HPLC provides remarkable separations of C$_{27}$ sterols differing only in the number or location of olefinic double bonds. This technique has been extended to LC-MS, analysis of purified components by GC, GC-MS, and $^{1}$H NMR, and to its use on a semipreparative scale. The application of this methodology for the demonstration of the catalysis, by rat liver microsomes, of the conversion of 7-dehydrocholesterol to cholesta-5,8-dien-3$\beta $-ol is also presented.

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