You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Direct Measurements of Kinesin Torsional Properties Reveal Flexible Domains and Occasional Stalk Reversals during Stepping
Braulio Gutiérrez-Medina, Adrian N. Fehr, Steven M. Block and James A. Spudich
Proceedings of the National Academy of Sciences of the United States of America
Vol. 106, No. 40 (Oct. 6, 2009), pp. 17007-17012
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/40485127
Page Count: 6
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Kinesin is a homodimeric motor with two catalytic heads joined to a stalk via short neck linkers (NLs). We measured the torsional properties of single recombinant molecules by tracking the thermal angular motions of fluorescently labeled beads bound to the C terminus of the stalk. When kinesin heads were immobilized on microtubules (MTs) under varied nucleotide conditions, we observed bounded or unbounded angular diffusion, depending on whether one or both heads were attached to the MT. Free rotation implies that NLs act as swivels. From data on constrained diffusion, we conclude that the coiled-coil stalk domains are ≈30-fold stiffer than its flexible "hinge" regions. Surprisingly, while tracking processive kinesin motion at low ATP concentrations, we observed occasional abrupt reversals in the directional orientations of the stalk. Our results impose constraints on kinesin walking models and suggest a role for rotational freedom in cargo transport.
Proceedings of the National Academy of Sciences of the United States of America © 2009 National Academy of Sciences