Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Systems Analysis of Plant Cell Wall Degradation by the Model Filamentous Fungus Neurospora crassa

Chaoguang Tian, William T. Beeson, Anthony T. Iavarone, Jianping Sun, Michael A. Marletta, Jamie H. D. Cate, N. Louise Glass and Arnold L. Demain
Proceedings of the National Academy of Sciences of the United States of America
Vol. 106, No. 52 (Dec. 29, 2009), pp. 22157-22162
Stable URL: http://www.jstor.org/stable/40536415
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Systems Analysis of Plant Cell Wall Degradation by the Model Filamentous Fungus Neurospora crassa
Preview not available

Abstract

The filamentous fungus Neurospora crassa is a model laboratory organism, but in nature is commonly found growing on dead plant material, particularly grasses. Using functional genomics resources available for N. crassa, which include a near-full genome deletion strain set and whole genome microarrays, we undertook a systemwide analysis of plant cell wall and cellulose degradation. We identified approximately 770 genes that showed expression differences when N. crassa was cultured on ground Miscanthus stems as a sole carbon source. An overlap set of 114 genes was identified from expression analysis of N. crassa grown on pure cellulose. Functional annotation of up-regulated genes showed enrichment for proteins predicted to be involved in plant cell wall degradation, but also many genes encoding proteins of unknown function. As a complement to expression data, the secretome associated with N. crassa growth on Miscanthus and cellulose was determined using a shotgun proteomics approach. Over 50 proteins were identified, including 10 of the 23 predicted N. crassa cellulases. Strains containing deletions in genes encoding 16 proteins detected in both the microarray and mass spectrometry experiments were analyzed for phenotypic changes during growth on crystalline cellulose and for cellulase activity. While growth of some of the deletion strains on cellulose was severely diminished, other deletion strains produced higher levels of extracellular proteins that showed increased cellulase activity. These results show that the powerful tools available in N. crassa allow for a comprehensive system level understanding of plant cell wall degradation mechanisms used by a ubiquitous filamentous fungus.

Page Thumbnails

  • Thumbnail: Page 
[22157]
    [22157]
  • Thumbnail: Page 
22158
    22158
  • Thumbnail: Page 
22159
    22159
  • Thumbnail: Page 
22160
    22160
  • Thumbnail: Page 
22161
    22161
  • Thumbnail: Page 
22162
    22162