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The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells
Esther Gerber, Andréa Hemmerlin, Michael Hartmann, Dimitri Heintz, Marie-Andrée Hartmann, Jérôme Mutterer, Manuel Rodríguez-Concepción, Albert Boronat, Alain van Dorsselaer, Michel Rohmer, Dring N. Crowell and Thomas J. Bach
The Plant Cell
Vol. 21, No. 1 (Jan., 2009), pp. 285-300
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/40537476
Page Count: 16
You can always find the topics here!Topics: Plant cells, Proteins, Cell membranes, Biosynthesis, Terpenoids, Epidermal cells, Fluorescence, Diphosphates, Cell nucleus, Plants
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Protein farnesylation and geranylgeranylation are important posttranslational modifications in eukaryotic cells. We visualized in transformed Nicotiana tabacum Bright Yellow-2 (BY-2) cells the geranylgeranylation and plasma membrane localization of GFP-BD-CVIL, which consists of green fluorescent protein (GFP) fused to the C-terminal polybasic domain (BD) and CVIL isoprenylation motif from the Oryza sativa calmodulin, CaM61. Treatment with fosmidomycin (Fos) or oxoclomazone (OC), inhibitors of the plastidial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, caused mislocalization of the protein to the nucleus, whereas treatment with mevinolin, an inhibitor of the cytosolic mevalonate pathway, did not. The nuclear localization of GFP-BD-CVIL in the presence of MEP pathway inhibitors was completely reversed by all-trans geranylgeraniol (GGol). Furthermore, 1-deoxy-D-xylulose (DX) reversed the effects of OC, but not Fos, consistent with the hypothesis that OC blocks 1-deoxy-D-xylulose 5-phosphate synthesis, whereas Fos inhibits its conversion to 2-C-methyl-Derythritol 4-phosphate. By contrast, GGol and DX did not rescue the nuclear mislocalization of GFP-BD-CVIL in the presence of a protein geranylgeranyltransferase type 1 inhibitor. Thus, the MEP pathway has an essential role in geranylgeranyl diphosphate (GGPP) biosynthesis and protein geranylgeranylation in BY-2 cells. GFP-BD-CVIL is a versatile tool for identifying Pharmaceuticals and herbicides that interfere either with GGPP biosynthesis or with protein geranylgeranylation.
The Plant Cell © 2009 American Society of Plant Biologists (ASPB)